세부품명번호4111540101세부품명형광광도계물품규격규격: 1. Platform (Main system:단백질 열 및 광산란 안정성 분석장비))
1) Instrument
- Simultaneous samples per experiment: 96
- Time to read a 96-well sample plate: 1 min
- Temperature range: 15–95 °C
- Heating rate: 0.1–10 °C/min
- Temperature accuracy: ±0.1 °C
- Laser classification: Class 1
2) Fluorescence
- Parameters determined: Tm, Tonset, Tm of vector payload ejection, rate of isothermal unfolding
- Excitation: 280 nm (UV) LED, 470 nm (blue) LED
- Detection: Spectrometer at full 250–750 nm spectral range
- Compatibility: Protein intrinsic fluorescence, Reporter fluorescence (DSF, nucleotide dye, membrane protein dye)
- Sample precision: <2% CV (Tm)
- Protein sample concentration: 0.025–300 mg/ml IgG (protein dependent)
3) Light scattering
- Parameters determined: Tagg, Tsize, rate of isothermal aggregation, Z-average diameter, size distributions, - polydispersity, colloidal stability(kD, B22, G22)
- Excitation: 660 nm (red) laser
- Detection: Avalanche photodiode
- SLS resolution: ≥15 kDa change of mean MW
- DLS hydrodynamic diameter range: 0.3–1000 nm
- DLS Size accuracy: ±2%
- Molecular weight range: 192 Da–25 MDa
- Minimum sample concentration: SLS: 0.05–300 mg/mL IgG (protein dependent), DLS: ≥0.1 mg/mL lysozyme
2. Workstation with software (분석용 워크스테이션 및 전용 소프트웨어)
1) version 1.2 software
- Export graphs to a file or directly to clipboard
- Noise filter applied to fluorescence and SLSdata for better data visualization
- Sample legends added to data overlay plots물품수량1 sys물품단가319,000,000원납품일수30일물품분류 제한여부아니오제조여부아니오배정예산금액319,000,000원 